Cell-Based Antioxidant Assay: Global Bio-Efficacy Testing for Pharmaceuticals, Nutraceuticals, & Cosmetics
Validate Your Compound with the Industry Standard
In the global market, proving “antioxidant activity” requires more than a chemical test. Leading regulatory bodies and Scopus-indexed journals now demand biological proof.
ACME Research Solutions delivers premium cell-based antioxidant assay services to clients worldwide. We provide the biological data you need to bridge the gap between benchtop chemistry and clinical trials.
Why Global Researchers Choose Our Cell-Based Antioxidant Assay
Standard chemical assays (DPPH/ORAC) are static. They cannot predict how a compound behaves inside a human body.
We utilize the Cellular Antioxidant Activity (CAA) assay—the most biologically relevant method recognized by the international scientific community.
Our analysis determines:
- Cellular Uptake: Does your active ingredient actually enter the cell?
- Bioavailability: Does it survive metabolic processing?
- Mechanism of Action: Differentiating between direct ROS scavenging and Nrf2 pathway activation.
Our Methodology: The Cell-Based Antioxidant Assay (CAA) Assay
We follow OECD guidelines and standard operating procedures to ensure your data is accepted globally.
1. Validated Cell Lines We match the cell line to your product’s target application:
- HepG2 (Liver): The global standard for bioavailability studies.
- Caco-2 (Gut): For digestive absorption and nutraceutical claims.
- HaCaT / Fibroblasts (Skin): For cosmetic anti-aging and anti-pollution claims.
2. The Protocol (DCFH-DA Method) We use the internationally standardized Dichlorofluorescin diacetate (DCFH-DA) probe method to measure intracellular ROS (reactive oxygen species) scavenging in real-time.
3. Deliverables
- EC50 / IC50 Calculations
- Publication-Quality Figures (300 DPI)
- Cytotoxicity Data (MTT/SRB) to rule out false positives.
International Sample Submission: Cell-Based Antioxidant Assay
We serve clients across Europe, North America, and Asia. Our logistics team makes sending samples easy.
- Customs Support: We provide the necessary HS Codes and “Non-Hazardous/For Research Use Only” declarations to ensure your samples clear customs smoothly.
- Courier Partners: We work seamlessly with FedEx, DHL, and World Courier.
- Small Sample Requirement: We only require 10-50 mg of your compound, making international shipping inexpensive.
Applications of Our Services
- Nutraceuticals: Validate herbal extracts and dietary supplements.
- Cosmetics: Prove anti-aging and anti-pollution claims for skincare formulations.
- Pharmaceuticals: Screen drug candidates for oxidative stress-related pathologies.
- Food Science: Test the stability and bio-efficacy of functional food ingredients.
Industries We Serve
- Pharma R&D: Preclinical screening for oxidative stress disorders.
- Nutraceuticals: Substantiating health claims for herbal extracts.
- Cosmetics: Validating “Anti-Pollution” and “Blue Light Protection” claims.
Ready to Elevate Your Research?
Don’t let your data be questioned. Get globally valid results from a specialized research partner.
ACME Research Solutions
- Service: Cell-Based Antioxidant Assays & Bio-Analysis
- Email: info@acmeresearchlabs.in
- Website: www.acmeresearchlabs.in
Frequently Asked Questions (FAQ)
Q: How much sample is required for a cell-based antioxidant assay? A: Typically, we require 10-50mg of the compound or extract, depending on its solubility and potency.
Q: Can you test lipophilic (oil-based) compounds? A: Yes, we utilize specialized nano-emulsion and delivery protocols to test lipophilic substances in cell culture media.
Q: Do you provide a comparison with standard antioxidants? A: Yes, all assays include a positive control (typically quercetin, vitamin C, or Trolox) to benchmark your product’s performance.
