Nanoemulsion Formulation & Development Services—At ACME Research Solutions, we approach formulation with engineering precision. Our laboratory specializes in converting BCS Class II and IV drugs (low solubility/high permeability) into thermodynamically stable, translucent, isotropic nanoemulsion and SEDDS systems.
We utilize our proprietary ACME BBD Optimization Engine and Nanoemulsion calculator (95% success)—specifically coded for lipid-based systems—to validate every variable before production, ensuring high reliability for Scopus-indexed research.
Nanoemulsion Formulation Services
Phase 1: Excipient Screening & Solubility Profiling
Successful formulation begins with precise vehicle selection. We conduct rigorous solubility screening of specific lipid vehicles and surfactants based on the HLB (hydrophilic-lipophilic balance) value required for your specific API.
Standard Reagent Inventory (Grade: Analytical/Pharmaceutical):
| Component Type | Chemicals / Trade Names Used | Selection Criteria |
|---|---|---|
| Oils (Lipids) | Capryol 90, Labrafil M 2125 CS, IPM, Oleic Acid, Castor Oil | Max API solubility (>10 mg/mL) |
| Surfactants | Tween 80, Cremophor EL, Labrasol, Span 20 | HLB value matching the oil phase |
| Co-Surfactants | Transcutol P, PEG 400, Propylene Glycol, Ethanol | Reduction of interfacial tension |
Phase 2: Construction of Pseudo-Ternary Phase Diagrams
To determine the precise nanoemulsion region, we perform detailed aqueous titration studies to map the boundary between turbid and clear regions.
- Smix Ratios Mapped: 1:1, 2:1, 3:1, and 4:1 (Surfactant to Co-surfactant).
- Method: Water titration method at ambient temperature 25°C±2°C.
- Data Density: Minimum 15-20 points per phase diagram to ensure accurate area calculation.
Phase 3: High-Energy Emulsification Process
For Nanoemulsion Formulation & Development Services, we employ high-shear homogenization followed by probe ultrasonication to fracture globules down to the nanometric scale (<100 nm).
Standard Operating Parameters (Optimizable per Project):
| Process Step | Equipment/Method | Parameter Settings |
|---|---|---|
| Coarse Emulsion | Magnetic Stirring | 750 – 1000 RPM for 30 mins |
| Droplet Reduction | Probe Ultrasonication | Frequency: 20-25 kHz |
| Cycle Settings | Pulse Mode (On/Off) | 5 sec ON / 5 sec OFF (Heat Dissipation) |
| Temp Control | Ice Bath Jacket | Maintain sample <30°C. |
Phase 4: Proprietary Statistical Optimization (ACME-BBD)
For Nanoemulsion Formulation & Development Services, we utilize our in-house developed ACME Nano-BBD Calculator to generate precise Surface Response Plots and Contour Plots. This tool is calibrated specifically for nano-formulations to streamline the optimization process.
Optimization Matrix:
- Design Type: Box-Behnken Design (3-Factor, 3-Level).
- Independent Variables X: Oil Concentration, Smix Concentration, and Sonication Amplitude.
- Dependent Variables Y:
- Particle Size Y1: Target <100 nm
- PDI Y2: Target <0.300 (Monodisperse)
- Zeta Potential Y3: Target ±30 mV
(Note: We provide access to our Free BBD Calculator for student verification and learning purposes)
Phase 5: Deliverables & Raw Data
We provide comprehensive data packages suitable for thesis submission and publication support.
| Analysis Type | Output Format Provided | Instrumentation Details |
|---|---|---|
| Droplet Size & PDI | .dts (raw), .pdf, .csv | Malvern Zetasizer |
| Zeta Potential | Peak Analysis Graph, Mean mV | Electrophoretic Light Scattering |
| Surface Morphology | High-Res .TIFF / .JPEG | TEM (Transmission Electron Microscopy) |
| Viscosity | Rheogram (Shear rate vs Stress) | Brookfield Viscometer |
| Drug Content | Calibration Curve + % Entrapment | HPLC / UV-Vis Spectrophotometer |
Thermodynamic Stability Testing Protocols
To ensure formulation integrity, samples undergo standard stress testing protocols.
- Centrifugation: 3,500 RPM for 30 minutes.
- Heating-Cooling Cycles: Six cycles between 4°C and 45°C.
- Freeze-Thaw Cycles: Three cycles between −21°C and +25°C.
Development Timeline: Standard lead time is 10-14 working days for full optimization and characterization.
Ready to start?
Send your API details and target dosage form to the ACME Research Solutions lab team.
