In Vitro Research Services: ACME Research Solutions delivers in vitro research services for pharmaceutical, biotech, and academic projects—built for reliable, report-ready data and fast coordination.
Our in vitro assay services support early screening, comparison studies, and manuscript-quality datasets for cell-based research.
Whether the requirement is cytotoxicity testing, cell migration, or oxidative stress screening, the workflow stays simple: share the study objective, confirm the plan, submit the samples, and receive a clean report with interpretable outputs.
Explore our in vitro services
Validate your drug candidates with publication-ready in vitro research services designed for reliability and speed. We provide audit-ready data for cytotoxicity, cell migration, and oxidative stress mechanisms.
MTT Cytotoxicity Assay
Cell viability testing for IC50
- 8-point dose-response curve
- IC50 calculation & statistical analysis
- Report with publication-ready graphs
- Turnaround: 7-10 days
Wound Healing Assay
Quantify cell migration & wound closure.
- Comprehensive study design & execution
- Time-lapse imaging (0h, 24h, 48h)
- % wound closure analysis
- Comparative group testing
- Turnaround: 5-7 days
Custom In Vitro Package
Multi-assay support for thesis projects.
- MTT + Wound Healing + ROS/Antioxidant
- Comprehensive study design & execution
- Full manuscript-ready datasets
- Priority support & faster delivery
Antioxidant Mechanisms in Live Cells
Prove your formulation’s efficacy by quantifying real-time oxidative stress reduction directly within living cell models. These advanced In vitro Research Services provide the robust mechanistic evidence required for high-impact pharmaceutical publications.
DPPH Radical Scavenging Assay
Evaluate free radical scavenging activity of compounds and extracts.
- IC50 determination for antioxidant potential
- Comparative analysis with standard antioxidants
- Concentration-dependent activity profiling
- Turnaround: 5-7 days
Intracellular ROS Detection
Quantify reactive oxygen species levels in living cells using fluorescent probes.
- DCFDA or similar fluorescent probe method
- Flow cytometry or microplate reader analysis
- Oxidative stress induction & compound protection testing
- Turnaround: 7-10 days
Total Antioxidant Capacity (TAC)
Comprehensive antioxidant potential measurement using multiple radical systems.
- ABTS, FRAP, or phosphomolybdenum methods
- Quantitative antioxidant equivalents
- Multi-sample batch processing
- Turnaround: 5-7 days
Molecular Mechanism Services
Uncover the precise biological pathways triggered by your formulation using advanced flow cytometry and fluorescence techniques. Our In vitro Research Services provide deep mechanistic insights—from cell cycle arrest to mitochondrial dysfunction—that turn basic data into high-impact research findings.
Apoptosis (Annexin V/PI)
Programmed cell death from necrosis with high precision
- Annexin V-FITC / PI dual staining quantifies live, apoptotic, and necrotic cells.
- Confirms the “mechanism of death” for cancer drugs.
- Generates quadrant dot-plots for publication-grade figures
Cell Cycle Analysis
Quantify reactive oxygen species levels in living cells using fluorescent probes.
- Quantifies DNA content to map G0/G1, S, and G2/M phases.
- Identifies cell cycle arrest points induced by your formulation.
- Uses Propidium Iodide (PI) staining with RNAse treatment.
Mitochondrial Potential (JC-1)
Comprehensive antioxidant potential measurement using multiple radical systems.
- JC-1 dye reveals loss of membrane potential (Delta-Psi-M).
- Visualizes the “point of no return” in the apoptotic pathway.
- Differentiates healthy (red aggregates) vs. apoptotic (green monomers) cells.
In vitro services Research Workflow (4-Step Process)
1. Study Consultation
We define your study objectives, select appropriate cell lines (e.g., HeLa, HEK293, MCF-7), and finalize the experimental design (groups, concentrations, time points).
2. Sample Submission
You ship your test samples (extracts, nanoparticles, or synthesized drugs) with our standardized sample submission form. We confirm receipt and storage conditions immediately.
3. Laboratory Execution
Our scientists execute the assays under strict SOPs. This includes cell culture maintenance, treatment, incubation, and data acquisition (absorbance/fluorescence/microscopy).
4. Data & Reporting
You receive a comprehensive study report containing raw data, calculated results (IC50, statistical significance), and high-resolution images ready for manuscript submission.
