Cell Culture Medium Optimization- The primary aim of maintaining cell lines in high standard is to maximize the reproducibility and reliability of the experiment. The Cell culture is the process by which cell are grown under the controlled environment with appropriate nutrition e.g. cell culture… Read More »Cell Culture Medium Optimization Techniques
In vitro Research
An in vitro cytotoxicity study is an assay to determine the effect of a substance on cells in a culture. This type of test is used to screen for potential drugs and evaluate new chemicals’ toxicity. The most common method uses cell lines derived from… Read More »In vitro Cytotoxicity Study-ACME Research Solutions
Cytotoxicity Test by MTT Assay- MTT ((3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) test is used to measure the cellular metabolic activity in mammalian cells as an indicator of cell viability, proliferation and cytotoxicity. Yellow water soluble MTT is metabolically reduced in viable cells to a blue-violet insoluble formazan. The… Read More »Cytotoxicity Test by MTT Assay- Procedure, Analysis and Results
In vitro toxicity studies play an important role in the development of new drugs and therapies. They help to assess the potential toxicity of a compound before it is tested in humans, and can provide critical information about how a compound might act in the… Read More »What is the Importance of In vitro Toxicity studies ?